Protein_Domain

Part:BBa_K3730036

Designed by: Guanrui Huang   Group: iGEM21_ZJU-China   (2021-09-14)


anti-hGPC3-scfv,a single chain variable antibody of human GPC3 protein. pET32a(+)

anti-hGPC3-scfv,a single chain variable antibody of human GPC3 protein. Fused with fiber protein. This design allows combination to GPC3 receptor which specifcally exists on the surface of the hepatic cancer cell. Can be fixed into pET32a(+).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 247
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 672
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 247
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 247
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None